{"id":1163,"date":"2021-03-03T12:06:25","date_gmt":"2021-03-03T17:06:25","guid":{"rendered":"https:\/\/demo.irdq.ca\/expertises\/example-of-expertise\/microscopie-electronique-en-transmission-met-tem\/"},"modified":"2021-05-06T15:35:01","modified_gmt":"2021-05-06T19:35:01","slug":"transmission-electron-microscopy-tem","status":"publish","type":"page","link":"https:\/\/demo.irdq.ca\/en\/expertises\/example-of-expertise\/transmission-electron-microscopy-tem\/","title":{"rendered":"Transmission electron microscopy (TEM)"},"content":{"rendered":"

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Transmission electron microscopy (TEM)<\/h1>\n

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About<\/h2>\n

The IRDQ offers an extensive range of transmission electron microscopes (TEM) which, combined with the expertise developed by highly qualified professionals, can provide a solution to the most demanding problems: analysis of small metallic particles (catalysts), self-assembled peptide arrays, 3D reconstruction of biological structures\u2026<\/p>\n

A sample analyzed by TEM must first be prepared in thin strips approximately 200 nm thick; the resulting small volume allows the sample\u2019s analysis in transmission, including chemical and crystallographic analyses. The thin strips are prepared by dedicated equipment such as a focused ion beam, ultramicrotomes and cryogenic units (for the subsequent study of biological specimens).<\/p>\n

TYPES OF ANALYSIS<\/h3>\n

The TEM\u2019s high space resolution makes it possible to characterize stereological parameters such as the size, shape or spatial distribution of structures. Chemical analyses can be conducted by energy dispersion X-ray spectroscopy (EDS) as well as electron energy loss spectroscopy (EELS). The latter can also yield information on chemical links between atoms and allows thickness measurement.<\/p>\n

The JSM 2100-F transmission electron microscope makes such measurements possible. The electron beam, with a minimum diameter of just 0.2 nm lends itself to imaging with a resolution of 0.14 nm. This makes the observation of atoms in crystalline samples possible, and permits the study of new materials developed on an atomic scale.<\/p>\n

BIOLOGICAL ANALYSES<\/h3>\n

To preserve the integrity of biological samples, cryogenic units must be used for both the preparation of samples and electron microscopy. Three cryogenic electron microscopes are available; the most recent acquisition, the Titan Krios 300 kV Cryo FE STEM by FEI, is unique in Canada. It features a Gatan Imaging Filter (GIF) and a CCD 16 megapixel camera allowing the 3D reconstruction of images with near-atomic resolution, making the Krios unique in the fields of medicine and biology.<\/p>\n

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Some examples of projects:<\/h3>\n